serpine2 human Search Results


shrna clone set against human serpine2  (Genecopoeia)
N/A
OmicsLink™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA
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human serpine2 transcript variant 2 gene orf cdna clone expression plasmid, n-flag tag  (Sino Biological)
N/A
Full length Clone DNA of Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 with N terminal Flag tag.
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human glia derived nexin serpine2 elisa kit  (Cusabio)
94
Cusabio human glia derived nexin serpine2 elisa kit
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Human Glia Derived Nexin Serpine2 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serpine2+human/pmc12873423-366-10-17?v=Cusabio
Average 94 stars, based on 1 article reviews
human glia derived nexin serpine2 elisa kit - by Bioz Stars, 2026-08
94/100 stars
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anti-human serpine2 antibody  (Abnova)
90
Abnova anti-human serpine2 antibody
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Anti Human Serpine2 Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serpine2+human/pm21426587-60-44-52?v=Abnova
Average 90 stars, based on 1 article reviews
anti-human serpine2 antibody - by Bioz Stars, 2026-08
90/100 stars
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serpine2 human sirna oligo duplex  (OriGene)
90
OriGene serpine2 human sirna oligo duplex
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Serpine2 Human Sirna Oligo Duplex, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serpine2+human/origene___sr303504?v=OriGene
Average 90 stars, based on 1 article reviews
serpine2 human sirna oligo duplex - by Bioz Stars, 2026-08
90/100 stars
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serpine2 nm 001136528 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles SERPINE2 mGFP tagged Human serpin peptidase inhibitor clade E nexin plasminogen activator inhibitor type 1 member 2 SERPINE2 transcript variant 2 200ul 10 7 TU mL
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human serpine2 transcript variant 2 gene orf cdna clone expression plasmid, n-ha tag  (Sino Biological)
N/A
Full length Clone DNA of Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 with N terminal HA tag.
   Buy from Supplier
human serpine2 transcript variant 2 gene orf cdna clone expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 with C terminal GFPSpark tag.
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serpine2 nm 001136530 human tagged orf clone  (OriGene)
N/A
SERPINE2 GFP tagged Human serpin peptidase inhibitor clade E nexin plasminogen activator inhibitor type 1 member 2 SERPINE2 transcript variant 4
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serpine2 nm 001136530 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles SERPINE2 mGFP tagged Human serpin peptidase inhibitor clade E nexin plasminogen activator inhibitor type 1 member 2 SERPINE2 transcript variant 4 200ul 10 7 TU mL
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serpine2 human shrna plasmid kit  (OriGene)
N/A
SERPINE2 Human 4 unique 29mer shRNA constructs in lentiviral GFP vector
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human serpine2 protein lysate  (Innovative Research Inc)
N/A
Human SERPINE2 Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. This is a Recombinant Protein Lysate produced in HEK293T cells. This protein lysate can be reconsituted using SDS Sample Buffer. Once
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Image Search Results


A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that SERPINE2 mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).

Journal: Communications Biology

Article Title: Integrated methylome analysis identifies an epigenetically silenced tumor suppressor RASA4 in small cell lung cancer

doi: 10.1038/s42003-025-09440-7

Figure Lengend Snippet: A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that SERPINE2 mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).

Article Snippet: SERPINE2 concentration in cell culture medium was quantified using the Human Glia-Derived Nexin (SERPINE2) ELISA Kit (#CSB-EL021082HU, Cusabio, Wuhan, China) following the manufacturer’s protocol.

Techniques: Knockdown, Transfection, shRNA, Quantitative RT-PCR, Over Expression, Stable Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, Negative Control, Expressing, Tube Formation Assay

A Representative images of H&E staining in tumor and adjacent normal tissues from FFPE specimens of three surgical SCLC patients. B IHC staining of RASA4 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patients. The results show significantly reduced RASA4 expression in the tumor tissues compared to the adjacent normal tissues. C IHC staining of SERPINE2 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patient samples. The results show significantly increased SERPINE2 expression in the tumor tissues compared to the adjacent normal tissues. (scale bar = 20 μm for 40×, scale bar = 100 μm for 8×) ( D ) IHC staining shows that SERPINE2 expression is elevated in the invasive front of the SCLC tumor. (scale bar = 40 μm for 20×, scale bar = 200 μm for 5×) ( E ) IHC scores of RASA4 and SERPINE2 in tumor and adjacent normal tissues from 29 SCLC patients who underwent surgery ( n = 29, Mann–Whitney test). F Statistical analysis indicates the negative association between RASA4 and SERPINE2 expression in SCLC tumor and adjacent normal tissues ( n = 29, Chi-square test for R × C contingency tables). G Kaplan–Meier survival analysis of 29 SCLC patients who underwent surgery, stratified by RASA4 IHC scores in tumor tissues. The results show that SCLC patients with moderate scores of RASA4 expression have significantly better survival compared to those with weak scores. The statistical significance of the difference in survival was determined by the log-rank test.

Journal: Communications Biology

Article Title: Integrated methylome analysis identifies an epigenetically silenced tumor suppressor RASA4 in small cell lung cancer

doi: 10.1038/s42003-025-09440-7

Figure Lengend Snippet: A Representative images of H&E staining in tumor and adjacent normal tissues from FFPE specimens of three surgical SCLC patients. B IHC staining of RASA4 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patients. The results show significantly reduced RASA4 expression in the tumor tissues compared to the adjacent normal tissues. C IHC staining of SERPINE2 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patient samples. The results show significantly increased SERPINE2 expression in the tumor tissues compared to the adjacent normal tissues. (scale bar = 20 μm for 40×, scale bar = 100 μm for 8×) ( D ) IHC staining shows that SERPINE2 expression is elevated in the invasive front of the SCLC tumor. (scale bar = 40 μm for 20×, scale bar = 200 μm for 5×) ( E ) IHC scores of RASA4 and SERPINE2 in tumor and adjacent normal tissues from 29 SCLC patients who underwent surgery ( n = 29, Mann–Whitney test). F Statistical analysis indicates the negative association between RASA4 and SERPINE2 expression in SCLC tumor and adjacent normal tissues ( n = 29, Chi-square test for R × C contingency tables). G Kaplan–Meier survival analysis of 29 SCLC patients who underwent surgery, stratified by RASA4 IHC scores in tumor tissues. The results show that SCLC patients with moderate scores of RASA4 expression have significantly better survival compared to those with weak scores. The statistical significance of the difference in survival was determined by the log-rank test.

Article Snippet: SERPINE2 concentration in cell culture medium was quantified using the Human Glia-Derived Nexin (SERPINE2) ELISA Kit (#CSB-EL021082HU, Cusabio, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Immunohistochemistry, Expressing, MANN-WHITNEY